Molecular Oncology (May 2023)
Disclosing quantitative RT‐PCR raw data during manuscript submission: a call for action
- Andreas Untergasser,
- Jan Hellemans,
- Michael W. Pfaffl,
- Jan M. Ruijter,
- Maurice J. B. van denHoff,
- Mihnea P. Dragomir,
- Douglas Adamoski,
- Sandra Martha Gomes Dias,
- Rui Manuel Reis,
- Manuela Ferracin,
- Emmanuel Dias‐Neto,
- Ian Marsh,
- Mikael Kubista,
- Muller Fabbri,
- Ajay Goel,
- Ondřej Slabý,
- Erik Knutsen,
- Baoqing Chen,
- Massimo Negrini,
- Koshi Mimori,
- Martin Pichler,
- Maria Papatriantafyllou,
- Simone Anfossi,
- Thomas D. Schmittgen,
- Jim Huggett,
- Stephen Bustin,
- Jo Vandesompele,
- George A. Calin,
- for the HEROIC (tHe initiativE gRoup On qRT dIsClosure) Consortium
Affiliations
- Andreas Untergasser
- Zentrum für Molekulare Biologie der Universität Heidelberg Germany
- Jan Hellemans
- CellCarta Zwijnaarde Belgium
- Michael W. Pfaffl
- Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan Technical University of Munich Freising Germany
- Jan M. Ruijter
- Department of Medical Biology, Amsterdam UMC, location AMC University of Amsterdam The Netherlands
- Maurice J. B. van denHoff
- Department of Medical Biology, Amsterdam UMC, location AMC University of Amsterdam The Netherlands
- Mihnea P. Dragomir
- Institute of Pathology, Berlin Institute of Health Charité ‐ Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt‐Universität zu Berlin Germany
- Douglas Adamoski
- Brazilian Biosciences National Laboratory (LNBio) Brazilian Center for Research in Energy and Materials (CNPEM) Campinas Brazil
- Sandra Martha Gomes Dias
- Brazilian Biosciences National Laboratory (LNBio) Brazilian Center for Research in Energy and Materials (CNPEM) Campinas Brazil
- Rui Manuel Reis
- Molecular Oncology Research Center Barretos Cancer Hospital Brazil
- Manuela Ferracin
- Department of Medical and Surgical Sciences (DIMEC) University of Bologna Italy
- Emmanuel Dias‐Neto
- Laboratory of Medical Genomics Centro Internacional de Pesquisa, AC Camargo Cancer Center São Paulo Brazil
- Ian Marsh
- New South Wales Department of Primary Industries Elizabeth Macarthur Agricultural Institute Narellan Australia
- Mikael Kubista
- TATAA Biocenter AB Göteborg Sweden
- Muller Fabbri
- Center for Cancer and Immunology Research Children's National Hospital Washington DC USA
- Ajay Goel
- Department of Molecular Diagnostics and Experimental Therapeutics Beckman Research Institute of City of Hope Monrovia CA USA
- Ondřej Slabý
- Department of Biology, Faculty of Medicine, Central European Institute of Technology Masaryk University Brno Czech Republic
- Erik Knutsen
- Department of Medical Biology, Faculty of Health Sciences UiT‐The Arctic University of Norway Tromsø Norway
- Baoqing Chen
- Department of Radiation Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine Sun Yat‐sen University Cancer Center Guangzhou China
- Massimo Negrini
- Laboratory for Technologies of Advanced Therapies (LTTA), Department of Morphology, Surgery and Experimental Medicine University of Ferrara Italy
- Koshi Mimori
- Department of Surgery Kyushu University Beppu Hospital Japan
- Martin Pichler
- Division of Oncology, Department of Internal Medicine Medical University of Graz Austria
- Maria Papatriantafyllou
- Editorial Manager Molecular Oncology Cambridge UK
- Simone Anfossi
- Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center Houston TX USA
- Thomas D. Schmittgen
- Department of Pharmaceutics University of Florida College of Pharmacy Gainesville FL USA
- Jim Huggett
- National Measurement Laboratory LGC Teddington UK
- Stephen Bustin
- Medical Technology Research Centre Anglia Ruskin University Chelmsford Essex UK
- Jo Vandesompele
- CellCarta Zwijnaarde Belgium
- George A. Calin
- Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center Houston TX USA
- for the HEROIC (tHe initiativE gRoup On qRT dIsClosure) Consortium
- DOI
- https://doi.org/10.1002/1878-0261.13418
- Journal volume & issue
-
Vol. 17,
no. 5
pp. 713 – 717
Abstract
Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT‐qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT‐qPCR raw data. The Real‐time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.
Keywords
