Study of Alkaline phosphatase Inhibition by theophylline and trifluoperazine in vitro

Abstract

ABSTRACT The aim of this research is to study the inhibition effect of theophylline and trifluoperazine in vitro on alkaline phosphatase and determine the type of inhibitions. A series of concentrations of both theophylline and trifluoperazine have been prepared and test their effect by adding them with the alkaline phosphatase assay. After converting the data from absorbance into enzymatic rate and test these velocities against concentrations of substrates using Linweaver –Burk equation, the results showed that both of theophylline and trifluoperazine have inhibition effect and the type of inhibition is non competitive. Using of Dixon methods indicate that the Ki of theophylline is 4.55 mM while for trifluoperazine Ki and is 1.07 mM.

Keywords

• alkaline phosphatase
• inhibition
• theophylline
• trifluoperazine
• in vitro