Scientific Reports (Feb 2021)

Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city

  • Charmaine Lloyd,
  • Kai Heng Tan,
  • Kar Leong Lim,
  • Vimala Gana Valu,
  • Sarah Mei Ying Fun,
  • Teng Rong Chye,
  • Hui Min Mak,
  • Wei Xiong Sim,
  • Sarah  Liyana Musa,
  • Joscelyn Jun Quan Ng,
  • Nazurah Syazana Bte Nordin,
  • Nurhazlyn Bte Md Aidzil,
  • Zephyr Yu Wen Eng,
  • Punithavathy Manickavasagam,
  • Jen Yan New

DOI
https://doi.org/10.1038/s41598-021-84112-0
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 6

Abstract

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Abstract This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed for their predominance and distribution. A total of 17 different algal genera were identified (H = 2.8, EH = 0.6), of which Scenedesmus spp. and Chlorella spp. constituted 27.5% and 21.3% of isolates respectively, followed by Micractinium spp. (18.8%) and Chlamydomonas spp. (12.5%). We also report 16 new microalgal strains from this region. The data is important from an ecological and biotechnological perspective.