Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2

Sarah Willkomm; Leonhard Jakob; Kevin Kramm; Veronika Graus; Julia Neumeier; Gunter Meister; Dina Grohmann

doi:10.1038/s41467-022-31480-4

Nature Communications (Jul 2022)

Single-molecule FRET uncovers hidden conformations and dynamics of human Argonaute 2

Sarah Willkomm,
Leonhard Jakob,
Kevin Kramm,
Veronika Graus,
Julia Neumeier,
Gunter Meister,
Dina Grohmann

Affiliations

Sarah Willkomm: Institute of Microbiology & Archaea Centre, Single-Molecule Biochemistry Lab, University of Regensburg
Leonhard Jakob: Institute of Microbiology & Archaea Centre, Single-Molecule Biochemistry Lab, University of Regensburg
Kevin Kramm: Institute of Microbiology & Archaea Centre, Single-Molecule Biochemistry Lab, University of Regensburg
Veronika Graus: Laboratory for RNA Biology, University of Regensburg
Julia Neumeier: Laboratory for RNA Biology, University of Regensburg
Gunter Meister: Laboratory for RNA Biology, University of Regensburg
Dina Grohmann: Institute of Microbiology & Archaea Centre, Single-Molecule Biochemistry Lab, University of Regensburg

DOI: https://doi.org/10.1038/s41467-022-31480-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

Read online

Single-molecule FRET measurements provide detailed insights into the conformational states and dynamics of human Argonaute 2 that are required for its function at the core of the eukaryotic RNA silencing pathway.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal