Box–Behnken Design Based Development of UV-Reversed Phase High Performance Liquid Chromatographic Method for Determination of Ascorbic Acid in Tablet Formulations
Syed Najmul Hejaz Azmi,
Umaima Al Hoqani,
Juhaina Obaid Said Al Mamari,
Buthaina Mohamed Salim Al Mamari,
Balaqis Sultan Ali Rashid Al Jassasi,
Aziza Saleh Saif Al Rubaiai,
Nafisur Rahman,
Mohd Nasir,
Sk Manirul Haque,
Shah Alam Khan,
Qamar Uddin Ahmed,
Zainul Amiruddin Zakaria
Affiliations
Syed Najmul Hejaz Azmi
Applied Sciences Department (Chemistry Section), Higher College of Technology, University of Technology and Applied Sciences, P.O. Box 74, Muscat 133, Oman
Umaima Al Hoqani
Applied Sciences Department (Biology Section), Higher College of Technology, University of Technology and Applied Sciences, P.O. Box 74, Muscat 133, Oman
Juhaina Obaid Said Al Mamari
Applied Sciences Department (Chemistry Section), Higher College of Technology, University of Technology and Applied Sciences, P.O. Box 74, Muscat 133, Oman
Buthaina Mohamed Salim Al Mamari
Applied Sciences Department (Chemistry Section), Higher College of Technology, University of Technology and Applied Sciences, P.O. Box 74, Muscat 133, Oman
Balaqis Sultan Ali Rashid Al Jassasi
Applied Sciences Department (Chemistry Section), Higher College of Technology, University of Technology and Applied Sciences, P.O. Box 74, Muscat 133, Oman
Aziza Saleh Saif Al Rubaiai
Applied Sciences Department (Chemistry Section), Higher College of Technology, University of Technology and Applied Sciences, P.O. Box 74, Muscat 133, Oman
Nafisur Rahman
Department of Chemistry, Aligarh Muslim University, Aligarh 202001, Uttar Pradesh, India
Mohd Nasir
Department of Chemistry, Aligarh Muslim University, Aligarh 202001, Uttar Pradesh, India
Sk Manirul Haque
Department of Chemical Engineering, Jubail Industrial College, Jubail Industrial City 31961, Saudi Arabia
Shah Alam Khan
Department of Pharmaceutical Chemistry, College of Pharmacy, National University of Science and Technology, P.O. Box 620, Muscat 130, Oman
Qamar Uddin Ahmed
Drug Discovery and Synthetic Chemistry Research Group, Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Kuantan 25200, Pahang Darul Makmur, Malaysia
Zainul Amiruddin Zakaria
Borneo Research on Algesia, Inflammation and Neurodegeneration (BRAIN) Group, Department of Biomedical Sciences, Faculty of Medicines and Health Sciences, University Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
A simple, sensitive, accurate and inexpensive UV-reversed-phase high-performance liquid chromatographic method was developed for the determination of ascorbic acid in tablet formulations. The method was based on the separation of ascorbic acid using a mobile phase of an acetonitrile-NaH2PO4-H3PO4 buffer solution (pH = 3) (5:95 v/v) with a UV detection wavelength of 245 nm and a flow rate of 0.8 mL min−1 at ambient column temperature. The variables of the proposed method, such as acetonitrile fraction (%), flow rate (mL min−1) and column temperature (°C), were optimized on the peak area by response surface methodology via the Box–Behnken design. The mobile phase was passed isocratically, and the separation of ascorbic acid was performed at the retention time of 4.1 min. A calibration graph was obtained and found to be linear in the concentration range of 10–180 µg mL−1. The method suitability was assessed and an asymmetry factor of 1.15 was obtained. The proposed method was successfully applied for the determination of ascorbic acid in tablet formulations and statistically compared with the results of the reference method. The performance of the proposed method was excellent and in agreement with the reference method. The recovery percentage of the proposed and reference methods was in the range of 99.98–100.04% and showed compliance (100 ± 2%) with regulatory guidelines.
