In sloped and intensively managed land, the soil characteristics are influenced mainly by water erosion intensity. In the present study, we evaluate the characteristics of Chernozems damaged by long-acting water erosion, particularly their retention and infiltration properties and possible impacts on soil fertility. Using infiltration experiments and a collection of intact samples, we performed analyses of the physical soil properties in individual transects. Our results confirm the lower infiltration capacity of deteriorated soil in the accumulation slope parts, which corresponded with the analyses of soil samples. The reduced bulk density in the accumulation slope parts exceeded 1.5 g·cm−3, indicating unsatisfactory (non-structured) soil conditions. In the transportation and eluvial slope zones, porosity values reached satisfactory numbers only at a depth of 10 cm. The median values of aeration showed a similar trend, but we recorded a higher value fluctuation.