Genetic diversity analysis of Centella asiatica L. Urban in Vietnam with RAPD marker

Abstract

Centella asiatica (L.) Urb., belonging to the Apiaceae family, grows widely in shady, moist or marshy places. C. asiatica has been used in traditional medical systems in Asia for over 100 years. A better understanding of genetic diversity is essential for its conservation and use. In this study, we performed the genetic diversity analysis for 24 cultivars of Centella asiatica using 24 selected RAPD markers. There are genetic differences among the studied samples. However, the molecular screening with RAPD markers shows that genetic difference (0.0025–0.2194) and genetic diversity indices (%P = 42.02%, h = 0.1272, I = 0.1938) are relatively low. Besides, HUIB_CA06 and HUIB_CA26 (from Thua Thien Hue province) are genetically different from the remaining varieties, which warrants further analysis of these varieties.

Keywords

• Centella asiatica
• Apiaceae family
• RAPD
• genetic diversity
• Vietnam