Cryo-EM structure of human Pol κ bound to DNA and mono-ubiquitylated PCNA

Claudia Lancey; Muhammad Tehseen; Souvika Bakshi; Matthew Percival; Masateru Takahashi; Mohamed A. Sobhy; Vlad S. Raducanu; Kerry Blair; Frederick W. Muskett; Timothy J. Ragan; Ramon Crehuet; Samir M. Hamdan; Alfredo De Biasio

doi:10.1038/s41467-021-26251-6

Nature Communications (Oct 2021)

Cryo-EM structure of human Pol κ bound to DNA and mono-ubiquitylated PCNA

Claudia Lancey,
Muhammad Tehseen,
Souvika Bakshi,
Matthew Percival,
Masateru Takahashi,
Mohamed A. Sobhy,
Vlad S. Raducanu,
Kerry Blair,
Frederick W. Muskett,
Timothy J. Ragan,
Ramon Crehuet,
Samir M. Hamdan,
Alfredo De Biasio

Affiliations

Claudia Lancey: Leicester Institute of Structural & Chemical Biology and Department of Molecular & Cell Biology, University of Leicester
Muhammad Tehseen: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology
Souvika Bakshi: Leicester Institute of Structural & Chemical Biology and Department of Molecular & Cell Biology, University of Leicester
Matthew Percival: Leicester Institute of Structural & Chemical Biology and Department of Molecular & Cell Biology, University of Leicester
Masateru Takahashi: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology
Mohamed A. Sobhy: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology
Vlad S. Raducanu: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology
Kerry Blair: Leicester Institute of Structural & Chemical Biology and Department of Molecular & Cell Biology, University of Leicester
Frederick W. Muskett: Leicester Institute of Structural & Chemical Biology and Department of Molecular & Cell Biology, University of Leicester
Timothy J. Ragan: Leicester Institute of Structural & Chemical Biology and Department of Molecular & Cell Biology, University of Leicester
Ramon Crehuet: CSIC-Institute for Advanced Chemistry of Catalonia (IQAC) C/ Jordi Girona 18-26
Samir M. Hamdan: Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology
Alfredo De Biasio: Leicester Institute of Structural & Chemical Biology and Department of Molecular & Cell Biology, University of Leicester

DOI: https://doi.org/10.1038/s41467-021-26251-6
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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Translesion Synthesis is a process that enables cells to overcome the deleterious effects of replication stalling caused by DNA lesions. Here the authors present a Cryo-EM structure of human Y-family DNA polymerase k (Pol k) bound to PCNA, P/T DNA and an incoming nucleotide; and propose a model for polymerase switching in which “carrier state” Pol k is recruited to PCNA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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