Evaluation of BSK-H Complete Medium Supplemented with Rabbit Serum and Sodium Bicarbonate for the Growth of Borrelia anserina

Abstract

The present study was conducted to compare the effect of 3 formulations Barbour-Stoenner-Kelly (BSK) medium on the growth of Borrelia (B.) anserina, the causative agent of avian borreliosis. Three different formulations of BSK medium (BSK-H, BSK-II and BSK-H complete medium) were prepared. For the isolation of B. anserina, Argas ticks were inoculated in all the three (BSK-H, BSK-II and BSK-H complete) formulations of BSK medium. All the samples were also observed for the impact of BSK medium on the growth (Generation time, Growth per hour, Specific growth rate) of B. anserina. Phase contrast microscopy was performed for the observation of viable B. anserina cells, and additional confirmation of all the isolates was done by performing indirect immunofluorescence assay and PCR. BSK-H complete medium supplemented with 6% rabbit serum and sodium bicarbonate was found best when compared to two other formulations of BSK medium with respect to the isolation, generation time and growth rate of Borrelia spirochetes.

Keywords

• Borrelia anserine
• BSK medium
• Fowl spirochaetosis