Inflammation and NFκB activation is decreased by hypothermia following global cerebral ischemia

Carla M. Webster; Stephen Kelly; Maya A. Koike; Valerie Y. Chock; Rona G. Giffard; Midori A. Yenari

Neurobiology of Disease (Feb 2009)

Inflammation and NFκB activation is decreased by hypothermia following global cerebral ischemia

Carla M. Webster,
Stephen Kelly,
Maya A. Koike,
Valerie Y. Chock,
Rona G. Giffard,
Midori A. Yenari

Affiliations

Carla M. Webster: Department of Neurology, University of California, San Francisco and the San Francisco Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, USA; Department of Anesthesia, Stanford University School of Medicine, Stanford, CA 94305, USA
Stephen Kelly: Clinical Sciences at South Bristol, University of Bristol, Bristol, BS1 3NY, UK
Maya A. Koike: Department of Neurology, University of California, San Francisco and the San Francisco Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, USA; Department of Anesthesia, Stanford University School of Medicine, Stanford, CA 94305, USA
Valerie Y. Chock: Department of Anesthesia, Stanford University School of Medicine, Stanford, CA 94305, USA
Rona G. Giffard: Department of Anesthesia, Stanford University School of Medicine, Stanford, CA 94305, USA
Midori A. Yenari: Department of Neurology, University of California, San Francisco and the San Francisco Veterans Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, USA; Corresponding author. Fax: +1 415 750 2273.

Journal volume & issue: Vol. 33, no. 2
pp. 301 – 312

Abstract

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We previously showed that hypothermia attenuates inflammation in focal cerebral ischemia (FCI) by suppressing activating kinases of nuclear factor-kappa B (NFκB). Here we characterize the inflammatory response in global cerebral ischemia (GCI), and the influence of mild hypothermia. Rodents were subjected to GCI by bilateral carotid artery occlusion. The inflammatory response was accompanied by microglial activation, but not neutrophil infiltration, or blood brain barrier disruption. Mild hypothermia reduced CA1 damage, decreased microglial activation and decreased nuclear NFκB translocation and activation. Similar anti-inflammatory effects of hypothermia were observed in a model of pure brain inflammation that does not cause brain cell death. Primary microglial cultures subjected to oxygen glucose deprivation (OGD) or stimulated with LPS under hypothermic conditions also experienced less activation and less NFκB translocation. However, NFκB regulatory proteins were not affected by hypothermia. The inflammatory response following GCI and hypothermia's anti-inflammatory mechanism is different from that observed in FCI.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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