Nature Communications (Mar 2021)

Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging

  • Henry Pinkard,
  • Hratch Baghdassarian,
  • Adriana Mujal,
  • Ed Roberts,
  • Kenneth H. Hu,
  • Daniel Haim Friedman,
  • Ivana Malenica,
  • Taylor Shagam,
  • Adam Fries,
  • Kaitlin Corbin,
  • Matthew F. Krummel,
  • Laura Waller

DOI
https://doi.org/10.1038/s41467-021-22246-5
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 14

Abstract

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Multiphoton microscopy requires precise increases in excitation power with imaging depth to generate contrast without damaging the sample. Here the authors show how an adaptive illumination function can be learned from the sample’s shape and used for in vivo imaging of whole lymph nodes.