Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量)

ZHENGQiang(郑强); RUANHong(阮红); XUZhi-nan(徐志南); YANGWei-jun(杨卫军)

doi:10.3785/j.issn.1008-9497.2008.06.020

Zhejiang Daxue xuebao. Lixue ban (Nov 2008)

Significantly improving the productivity of glutamine with over-expression of glnA in Corynebacterium glutamicum(谷氨酰胺合成酶基因的过量表达有效提高谷氨酸棒杆菌中L-谷氨酰胺产量)

ZHENGQiang(郑强),
RUANHong(阮红),
XUZhi-nan(徐志南),
YANGWei-jun(杨卫军)

Affiliations

ZHENGQiang(郑强): 1.College of Life Sciences, Zhejiang University, Hangzhou 310058, China( 1.浙江大学生命科学学院，浙江杭州 310058)
RUANHong(阮红): 2.College of Medical and Life Science, City College of Zhejiang University, Hangzhou 310015, China( 2.浙江大学城市学院医学与生命科学学院，浙江杭州 310015)
XUZhi-nan(徐志南): 3.College of Material Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China( 3.浙江大学材料与化学工程学院，浙江杭州 310027)
YANGWei-jun(杨卫军): 1.College of Life Sciences, Zhejiang University, Hangzhou 310058, China( 1.浙江大学生命科学学院，浙江杭州 310058)

DOI: https://doi.org/10.3785/j.issn.1008-9497.2008.06.020
Journal volume & issue: Vol. 35, no. 6
pp. 678 – 683

Abstract

Read online

以谷氨酸棒杆菌Corynebacterium glutamicum ATCC13032基因组为模板，通过PCR扩增，得到大小为1434bp的谷氨酰胺合成酶基因glnA.以大肠杆菌/谷氨酸棒杆菌穿梭质粒pXMJ19为载体，将扩增得到的目的基因片段克隆至C. glutamicum Res167，获得重组菌株C. glutamicum Res167/pXMJ19-glnA.在摇瓶发酵水平上，通过IPTG诱导glnA基因的表达，并采用反相高效液相色谱方法测定了发酵液中的L-谷氨酰胺含量.结果显示，与未经诱导的对照菌相比，诱导后的重组菌发酵液中的L-谷氨酰胺产量提高了1.8倍，最高产量达1.54 g• L-1.

Published in Zhejiang Daxue xuebao. Lixue ban

ISSN: 1008-9497 (Print)
Publisher: Zhejiang University Press
Country of publisher: China
LCC subjects: Science: Mathematics: Instruments and machines: Electronic computers. Computer science; Science: Physics
Website: https://www.zjujournals.com/sci/EN/1008-9497/home.shtml

About the journal

Abstract

Keywords