Vaccines (Mar 2023)

Using an Aluminum Hydroxide–Chitosan Matrix Increased the Vaccine Potential and Immune Response of Mice against Multi-Drug-Resistant <i>Acinetobacter baumannii</i>

  • Túllio T. Deusdará,
  • Mellanie K. C. Félix,
  • Helio de S. Brito,
  • Edson W. S. Cangussu,
  • Wellington de S. Moura,
  • Benedito Albuquerque,
  • Marcos G. Silva,
  • Gil R. dos Santos,
  • Paula B. de Morais,
  • Elizangela F. da Silva,
  • Yury O. Chaves,
  • Luis Andre M. Mariúba,
  • Paulo A. Nogueira,
  • Spartaco Astolfi-Filho,
  • Enedina N. Assunção,
  • Sabrina Epiphanio,
  • Claudio R. F. Marinho,
  • Igor V. Brandi,
  • Kelvinson F. Viana,
  • Eugenio E. Oliveira,
  • Alex Sander R. Cangussu

DOI
https://doi.org/10.3390/vaccines11030669
Journal volume & issue
Vol. 11, no. 3
p. 669

Abstract

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Acinetobacter baumannii is a Gram-negative, immobile, aerobic nosocomial opportunistic coccobacillus that causes pneumonia, septicemia, and urinary tract infections in immunosuppressed patients. There are no commercially available alternative antimicrobials, and multi-drug resistance is an urgent concern that requires emergency measures and new therapeutic strategies. This study evaluated a multi-drug-resistant A. baumannii whole-cell vaccine, inactivated and adsorbed on an aluminum hydroxide–chitosan (mAhC) matrix, in an A. baumannii sepsis model in immunosuppressed mice by cyclophosphamide (CY). CY-treated mice were divided into immunized, non-immunized, and adjuvant-inoculated groups. Three vaccine doses were given at 0D, 14D, and 28D, followed by a lethal dose of 4.0 × 108 CFU/mL of A. baumannii. Immunized CY-treated mice underwent a significant humoral response, with the highest IgG levels and a higher survival rate (85%); this differed from the non-immunized CY-treated mice, none of whom survived (p p A. baumannii infections.

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