Frontiers in Plant Science (Aug 2021)

Alpha Carbonic Anhydrase 5 Mediates Stimulation of ATP Synthesis by Bicarbonate in Isolated Arabidopsis Thylakoids

  • Tatiana P. Fedorchuk,
  • Inga A. Kireeva,
  • Vera K. Opanasenko,
  • Vasily V. Terentyev,
  • Natalia N. Rudenko,
  • Maria M. Borisova-Mubarakshina,
  • Boris N. Ivanov

DOI
https://doi.org/10.3389/fpls.2021.662082
Journal volume & issue
Vol. 12

Abstract

Read online

We studied bicarbonate-induced stimulation of photophosphorylation in thylakoids isolated from leaves of Arabidopsis thaliana plants. This stimulation was not observed in thylakoids of wild-type in the presence of mafenide, a soluble carbonic anhydrase inhibitor, and was absent in thylakoids of two mutant lines lacking the gene encoding alpha carbonic anhydrase 5 (αCA5). Using mass spectrometry, we revealed the presence of αCA5 in stromal thylakoid membranes of wild-type plants. A possible mechanism of the photophosphorylation stimulation by bicarbonate that involves αCA5 is proposed.

Keywords