Comparative Analysis of <i>Campylobacter jejuni</i> and <i>C. coli</i> Isolated from Livestock Animals to <i>C. jejuni</i> and <i>C. coli</i> Isolated from Surface Water Using DNA Sequencing and MALDI-TOF
Martine Denis,
Valérie Rose,
Bérengère Nagard,
Amandine Thépault,
Pierrick Lucas,
Meagan Meunier,
Fabienne Benoit,
Amandine Wilhem,
Benoit Gassilloud,
Elodie Cauvin,
Alain Rincé,
Michèle Gourmelon
Affiliations
Martine Denis
Ploufragan-Plouzané-Niort Laboratory, Hygiene and Quality of Poultry and Pork Products Unit, ANSES (French Agency For Food, Environmental and Occupational Health and Safety), 22440 Ploufragan, France
Valérie Rose
Ploufragan-Plouzané-Niort Laboratory, Hygiene and Quality of Poultry and Pork Products Unit, ANSES (French Agency For Food, Environmental and Occupational Health and Safety), 22440 Ploufragan, France
Bérengère Nagard
Ploufragan-Plouzané-Niort Laboratory, Hygiene and Quality of Poultry and Pork Products Unit, ANSES (French Agency For Food, Environmental and Occupational Health and Safety), 22440 Ploufragan, France
Amandine Thépault
Ploufragan-Plouzané-Niort Laboratory, Hygiene and Quality of Poultry and Pork Products Unit, ANSES (French Agency For Food, Environmental and Occupational Health and Safety), 22440 Ploufragan, France
Pierrick Lucas
Ploufragan-Plouzané-Niort Laboratory, Viral Genetics and Biosafety Unit, ANSES, 22440 Ploufragan, France
Meagan Meunier
Caen-Saint-Lô Laboratory, Research Department, LABEO, 50000 Saint-Lô, France
Fabienne Benoit
Caen-Saint-Lô Laboratory, Research Department, LABEO, 50000 Saint-Lô, France
Amandine Wilhem
Nancy Laboratory, ANSES, PTF Maldi, 54000 Nancy, France
Benoit Gassilloud
Nancy Laboratory, ANSES, PTF Maldi, 54000 Nancy, France
Elodie Cauvin
Caen-Saint-Lô Laboratory, Research Department, LABEO, 50000 Saint-Lô, France
Alain Rincé
Bacterial Communication and Anti-Infectious Strategies Reseach Unit, UNICAEN (Caen Normandie University), UR4312 CBSA, 14000 Caen, France
Michèle Gourmelon
ODE-DYNECO-PELAGOS (Department of Oceanography and Ecosystem Dynamics, Coastal Environment Dynamics and Pelagic Ecology Research Unit), IFREMER (French Research Institute for Exploitation of the Sea), 29280 Plouzané, France
This study evaluated the contribution of cattle, sheep, poultry and pigs to the contamination of surface water from rivers by Campylobacter jejuni and C. coli using MLST, cgMLST and considered MALDI-TOF MS as an alternative technique. The 263 strains isolated from cattle (n = 61), sheep (n = 42), poultry (n = 65), pigs (n = 60) and surface water (n = 35) were distributed across 115 sequence types (STs), 49 for C. jejuni and 66 for C. coli. Considering MLST data, 14.2%, 11.4% and 2.8% of the surface water strains could be attributed to cattle, poultry and sheep, respectively, none to pigs, and 85.7% were non-attributed. Analysis of cg-MLST data with STRUCTURE indicated that C. jejuni strains from water were predominantly attributed to poultry (93.5%), weakly to sheep (C. coli strains from water were predominantly non-attributed (94.3%) and 5.7% attributed to poultry. Considering the protein profiles with a threshold of 94% and 97% of similarity, respectively, strains from surface water could be attributed to poultry (31.4% and 17.1%), and to cattle (17.1% and 5.7%); 54.1% and 77.1% were non-attributed. This study confirmed these livestock animals might contribute to the contamination of surface water, with a level of contribution depending on the typing technique and the method of analysis. MALDI-TOF could potentially be an alternative approach for source attribution.
