Journal of Krishna Institute of Medical Sciences University (Jan 2018)
Role of Choline-Docosahexaenoic acid and Trigonella foenum graecum Seed Extract on Ovariectomy Induced Dyslipidemia and Oxidative Stress in Rat Model
Abstract
Background: Menopause is characterized by the deficiency of ovarian hormones, mainly estrogen. The decline in estrogen hormone is contributing the cardiovascular disorders in women. Hormone replacement therapy has disadvantages especially a higher risk of breast, ovarian and endometrial cancers upon chronic use. Phytoestrogens may be used as an alternative to hormone replacement therapy. Aim and Objectives: This study was designed to scientifically evaluate the role of Choline- Docosahexaenoic Acid (DHA) and Trigonella foenum graecum (TFG) seed extract on Ovariectomy (OVX) induced dyslipidemia and oxidative stress in rat model. Material and Methods: Female Wistar rats were allocated into four groups (n=6):1) Sham control, 2) ovariectomized, 3) ovariectomized+ choline-DHA and 4) ovariectomized + choline-DHA+TFG. After 30 days of treatment, fasting blood samples and liver tissues were collected. Serum was analyzed for lipid profile and liver homogenates were used for assessment of oxidative stress and antioxidant activity. Results: Ovariectomized rats showed significantly increased (P<0.05) Total Cholesterol (TC), Low Density Lipoprotein (LDL) levels and decreased High Density Lipoprotein (HDL) levels. Treating ovariectomized rats with choline-DHA and TFG seed extract significantly lowered (P<0.05) total cholesterol, LDL and markedly increased the HDL. Significantly increased (P≤0.01) Thiobarbituric Acid Reactive Substances (TBARS) and reduced (P<0.05) glutathione levels were observed in OVX group. The synergetic effect of choline-DHA and fenugreek showed a significant reduction ((P≤0.01) in TBARS levels. Conclusion: These results showed that choline-DHA with TFG supplementation have a favorable effect on OVX induced hyperlipidemia and oxidative stress. Therefore, these components may be a therapeutic agent for treating the menopause induced hyperlipidemia or oxidative stress.
