Investigation of hematological indices in experimental peritonitis

Abstract

Objective. To investigate hematological indices on the first, third and seventh day of the experimental peritonitis development. Маterials and methods. The investigation was conducted on 180 Wistar line rats with a body mass 180 - 200 g. The animals were divided into four Groups: Group I - 20 intact animals; Group II - 80 animals, in whom a biliary peritonitis was simulated without further correction; Group III - 40 animals, in whom the biliary peritonitis simulated was corrected, using abdominal cavity sanation with chlorhexidine solution and further application of standard antibioticotherapy; Group IV - 40 animals, in whom a simulated biliary peritonitis was corrected in accordance to combined scheme of detoxication. Results. While investigation of hematological markers in animals of Group IV, to whom complex treatment was applied, a positive dynamics of indices of the erythrocytes quantity, hemoglobin concentration, hematocrit and ERS were observed on the third day and preserved up to the seventh day. The indices of the leucocytes quantity more significantly had normalized on the first and third days. The leucocytic index of intoxication had lowered on the third day. Most pronounced positive dynamics of the AST activity was observed on the seventh day, and of the ALT - on the third. On the first and third day of experiment the least pronounced lowering of the general protein concentration was noted, and on the seventh day - its raise. Concentration of general bilirubin maximally approached the indices of intact animals on the third day. Conclusion. Investigation of hematological markers have proved the efficacy of our proposed method of the abdominal cavity sanation.

Keywords

• peritonitis; experimental model; sanation of abdominal cavity; decametoxin; sodium hyaluronate.