Resonating with Cellular Pathways: Transcriptome Insights into Nonthermal Bioeffects of Middle Infrared Light Stimulation and Vibrational Strong Coupling on Cell Proliferation and Migration
Xingkun Niu,
Zhongyu Wu,
Feng Gao,
Shaojie Hou,
Shihao Liu,
Xinmin Zhao,
Liping Wang,
Jun Guo,
Feng Zhang
Affiliations
Xingkun Niu
Quantum Biophotonic Lab, Key Laboratory of Optical Technology and Instrument for Medicine, Ministry of Education, School of Optical-Electrical and Computer Engineering,
University of Shanghai for Science and Technology, Shanghai 200093, China.
Zhongyu Wu
Department of Nuclear Medicine,
The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250013, China.
Feng Gao
Wenzhou Institute,
University of Chinese Academy of Sciences, Wenzhou 325001, China.
Shaojie Hou
Quantum Biophotonic Lab, Key Laboratory of Optical Technology and Instrument for Medicine, Ministry of Education, School of Optical-Electrical and Computer Engineering,
University of Shanghai for Science and Technology, Shanghai 200093, China.
Shihao Liu
Wenzhou Institute,
University of Chinese Academy of Sciences, Wenzhou 325001, China.
Xinmin Zhao
Quantum Biophotonic Lab, Key Laboratory of Optical Technology and Instrument for Medicine, Ministry of Education, School of Optical-Electrical and Computer Engineering,
University of Shanghai for Science and Technology, Shanghai 200093, China.
Liping Wang
Wenzhou Institute,
University of Chinese Academy of Sciences, Wenzhou 325001, China.
Jun Guo
Wenzhou Institute,
University of Chinese Academy of Sciences, Wenzhou 325001, China.
Feng Zhang
Quantum Biophotonic Lab, Key Laboratory of Optical Technology and Instrument for Medicine, Ministry of Education, School of Optical-Electrical and Computer Engineering,
University of Shanghai for Science and Technology, Shanghai 200093, China.
Middle infrared stimulation (MIRS) and vibrational strong coupling (VSC) have been separately applied to physically regulate biological systems but scarcely compared with each other, especially at identical vibrational frequencies, though they both involve resonant mechanism. Taking cell proliferation and migration as typical cell-level models, herein, we comparatively studied the nonthermal bioeffects of MIRS and VSC with selecting the identical frequency (53.5 THz) of the carbonyl vibration. We found that both MIRS and VSC can notably increase the proliferation rate and migration capacity of fibroblasts. Transcriptome sequencing results reflected the differential expression of genes related to the corresponding cellular pathways. This work not only sheds light on the synergistic nonthermal bioeffects from the molecular level to the cell level but also provides new evidence and insights for modifying bioreactions, further applying MIRS and VSC to the future medicine of frequencies.
