Molecular detection and phylogenetic analysis of Coxiella burnetii in goats milk

Abstract

Over the last years, the number Coxiella burnetii infections increased throughout the wourld. A molecular detection and phylogenetic analysis were performed in Al-Diwaniayh city in which the first phylogenetic documentation of C.burnetii reported in this area with accession numbers KY576802.1, KY576803.1, KY576804.1 and KY576805.1 Molecular detection and phylogenetic analysis of C.burnetii targeting the transposase gene partial sequence was done on the milk samples from 50 apperently healthy goats. DNA sequence was amplified in 6 out of 50 (12%) milk samples collected from different regions of Al Diwaniya city. Four of these amplicons were submitted to sequences analysis and gave 99% nucleotides sequence similarity. Also the amplicon sequences of local strain were compared with global C.burnetti sequences in NCBI which revealed close related to NCBI-Blast C.burnetii transposase gene of Indian (AB84899.1) and Brazilian (JF970261.1) strains, whereas there was genetic variation to NCBI-Blast C.burnetii transposase gene for USA (DQ379976.1), Portugal (EU009657.1) and Taiwan (EU000273.1). This work confirm goats infection and shedding the C. burnetii in Al-Diwaniayh, Iraq and determined the phylogenetic tree of local strain of this bacterium.