Chemical constituents of ethyl acetate extract from Corydalis saxicola and its anti-inflammatory activity

Abstract

Corydalis saxicola which belongs to the family of Papaveraceae, is always used to treat various diseases such as hepatitis B, cirrhosis, furuncle, acute icteric hepatitis, and advanced cancer as traditional Zhuang medicine in Guangxi province. To study the anti-inflammatory activity of the chemical constituents of C. saxicola, silica gel, MCI, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography were used to separate the chemical constituents from the ethyl acetate fraction of 95% ethanol extract of C. saxicola. Their structures were identified by nuclear magnetic resonance (NMR) and high resolution mass spectrum (HR-MS). In addition, the anti-inflammatory activity was evaluated by the mouse macrophage RAW264.7 induced by lipopolysaccharide (LPS). The results showed that 13 chemical compounds were isolated and identified as coptisine (1), berberine hydrochloride (2), crinumaquine (3), worenine (4), dehydrocavidine (5), (-)-tetrahydropalmatine (6), jatrorrhizine (7), corydaldine (8), N-trans-feruloyl tyramine (9), dibutylterephthalate (10), kaempferol (11), isololiolide (12), loliolide (13) respectively. Compounds 3, 4,8-13 were isolated from this plant for the first time. Compounds 4, 9 and 11 showed good inhibitory effects on LPS induced NO production by macrophage RAW264.7 with IC50 values of (18.8 ± 0.2), (29.1 ± 0.3), (18.0 ± 0.1) μmol·L-1 respectively, which was better than the positive control indomethacin, exhibited good potential in the research and development of anti-inflammatory drugs. These results can clarify the anti-inflammatory material basis of C. saxicola, and provide a scientific theoretical basis for its further utilization.

Keywords

• corydalis saxicola
• ethyl acetate extract
• chemical constituents
• isolation and identification
• anti-inflammatory activity