Differential Inductive Sensor for Continuous Non-Invasive Cell Growth Monitoring in Disposable Bioreactors
Maria Allers,
Tobias Reinecke,
Tamanna Nagraik,
Dörte Solle,
Kai Bakes,
Marc Berger,
Thomas Scheper,
Stefan Zimmermann
Affiliations
Maria Allers
Department of Sensors and Measurement Technology, Institute of Electrical Engineering and Measurement Technology, Leibniz Universität Hannover, Hannover, Germany
Tobias Reinecke
Department of Sensors and Measurement Technology, Institute of Electrical Engineering and Measurement Technology, Leibniz Universität Hannover, Hannover, Germany
Tamanna Nagraik
Institute of Technical Chemistry, Leibniz Universität Hannover, Hannover, Germany
Dörte Solle
Institute of Technical Chemistry, Leibniz Universität Hannover, Hannover, Germany
Kai Bakes
Department of Sensors and Measurement Technology, Institute of Electrical Engineering and Measurement Technology, Leibniz Universität Hannover, Hannover, Germany
Marc Berger
Department of Sensors and Measurement Technology, Institute of Electrical Engineering and Measurement Technology, Leibniz Universität Hannover, Hannover, Germany
Thomas Scheper
Institute of Technical Chemistry, Leibniz Universität Hannover, Hannover, Germany
Stefan Zimmermann
Department of Sensors and Measurement Technology, Institute of Electrical Engineering and Measurement Technology, Leibniz Universität Hannover, Hannover, Germany
In this work, we present a low-cost sensor system for continuous non-invasive cell growth monitoring, especially for single use bioreactor (SUB) applications. The sensor system is based on a differential transformer. Using this differential setup, the influence of the primary magnetic flux is eliminated from the measuring signal, enabling highly sensitive non-invasive detection of permittivity changes in the culture medium. To evaluate the sensor, E. coli cultivations are performed and the cell density is measured through the polymer foil of a SUB. We found a linear dependency with low data scattering between measuring signal and cell density.