PCR-SSCP of growth hormone gene and its association with body weight in Black Bengal goat

Abstract

Present study was undertaken to investigate the single nucleotide polymorphism within growth hormone gene and its correlation with body weight in Black Bengal goat. Two fragments of growth hormone gene, 245 bp fragment (partial intron 1, exon 2 and partial intron 2) and 472 bp fragment (partial intron 2, exon 3 and intron 3 and partial exon 4) were analyzed for detection of polymorphism expected to be present at this locus. SSCP of 245 bp and 472 bp fragment revealed 4 and 5 genotypes, respectively. Sequencing revealed substitution at 5 places in 245 bp fragment whereas at 6 places in 472 bp fragment of growth hormone gene. Least square analysis revealed that only 472 bp fragment genotypes had significant effect on body weight at 6 and 9 month of age. Animals having AC genotype had the highest birth weight, whereas animals having CC genotype had lowest birth weight.

Keywords

• Goat
• Growth hormone
• Polymorphism
• Sequencing