Evaluation of Analgesics and Anti-Inflammatory Activity of the Root Extract of Impatiens rothii (Balsaminaceae) in Rodents

Abstract

Getachew Ashagrie,1,2 Fentaw Girmaw,1 Abebe Tarekegn,1 Tenaw Baye,1 Abebe Dagne3 1Department of Pharmacy, College of Health Science, Woldia University, Woldia, Ethiopia; 2Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 3Department of Pharmacy, College of Health science, Debre Markos University, Debre Markos, EthiopiaCorrespondence: Getachew Ashagrie, Email [email protected]: The roots of Impatiens rothii has been used as a traditional remedy for painful conditions, rheumatism, isthmus and crural aches. However, the analgesic and anti-inflammatory properties of this plant have yet to be scientifically confirmed. The purpose of this study was to explore possible analgesic and anti-inflammatory activities 80% methanolic root extract of Impatiens rothii.Methods: To obtain the crude extract, the roots of Impatiens rothii that had been dried and ground up were macerated in 80% methanol. The analgesic activity was determined using acetic acid-induced writhing and hot plate tests in mice, whereas the anti-inflammatory activity was analyzed using carrageenan-induced paw edema model in rats. The extract was orally administered at a dose of 100, 200 and 400 mg/kg.Results: All tested doses of Impatiens rothii extract showed significant analgesic activity (p< 0.05) at observations of 30 to 120 minutes compared to the negative control in the hot plate test. In acetic acid-induced writhing test all tested doses of the 80% methanol extract of Impatiens rothii significantly (p < 0.001) reduced the number of writhing. In comparison to the control group, all tested doses displayed a significant decrease in paw edema, which appeared 2– 5 hours after induction (p< 0.05).Conclusion: From the results of this study, it can be stated that 80% methanolic extract of Impatiens rothii possessed substantial analgesic and anti-inflammatory activities, hence providing scientific basis for the use of this plant in the treatment of pain and inflammatory diseases.Keywords: Impatiens rothii, analgesic, hot plate, anti-inflammatory, carrageenan- induced paw edema

Keywords

• impatiens rothii
• analgesic
• hot plate
• anti-inflammatory
• carrageenan- induced paw edema