An Abraded Surface of Doxorubicin-Loaded Surfactant-Containing Drug Delivery Systems Effectively Reduces the Survival of Carcinoma Cells
Christian Schmidt,
Fabiano Yokaichiya,
Nurdan Doğangüzel,
Margareth K. K. Dias Franco,
Leide P. Cavalcanti,
Mark A. Brown,
Melissa I. Alkschbirs,
Daniele R. de Araujo,
Mont Kumpugdee-Vollrath,
Joachim Storsberg
Affiliations
Christian Schmidt
Department of Biomaterials and Healthcare, Division of Life Science and Bioprocesses, Fraunhofer Institute for Applied Polymer Research (IAP), Geiselbergstraße 69, 14476 Potsdam-Golm, Germany
Fabiano Yokaichiya
Institute of Materials and Energy, Helmholtz-Center Berlin, Hahn-Meitner-Platz 1, 14109 Berlin, Germany
Nurdan Doğangüzel
Department of Biomaterials and Healthcare, Division of Life Science and Bioprocesses, Fraunhofer Institute for Applied Polymer Research (IAP), Geiselbergstraße 69, 14476 Potsdam-Golm, Germany
Margareth K. K. Dias Franco
Instituto de Pesquisas Energéticas e Nucleares, Avenida Lineo Prestes, 2342, Cidade Universitária Armando Salles de Oliveira, SP 05508-900, Brazil
Leide P. Cavalcanti
School of Chemical Engineering, University of Campinas, Campinas, SP 13083-970, Brazil
Mark A. Brown
Department of Clinical Sciences, Cell and Molecular Biology Program and Flint Cancer Center, Colorado State University, Fort Collins, CO 80523-1052, USA
Melissa I. Alkschbirs
Instituto de Química, Campinas, Universidade Estadual de Campinas, Campinas, SP 13083-970, Brazil
Daniele R. de Araujo
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Santo André, SP 09210-580, Brazil
Mont Kumpugdee-Vollrath
Department of Pharmaceutical Engineering, Beuth University of Applied Sciences Berlin, 13353 Berlin, Germany
Joachim Storsberg
Department of Biomaterials and Healthcare, Division of Life Science and Bioprocesses, Fraunhofer Institute for Applied Polymer Research (IAP), Geiselbergstraße 69, 14476 Potsdam-Golm, Germany
An effective antitumor remedy is yet to be developed. All previous approaches for a targeted delivery of anticancer medicine have relied on trial and error. The goal of this study was to use structural insights gained from the study of delivery systems and malignant cells to provide for a systematic approach to the development of next-generation drugs. We used doxorubicin (Dox) liposomal formulations. We assayed for cytotoxicity via the electrical current exclusion method. Dialysis of the samples yielded information about their drug release profiles. Information about the surface of the delivery systems was obtained through synchrotron small-angle X-ray scattering (SAXS) measurements. SAXS measurements revealed that Dox-loading yielded an abraded surface of our Dox liposomal formulation containing soybean oil, which also correlated with an effective reduction of the survival of carcinoma cells. Furthermore, a dialysis assay revealed that a higher burst of Dox was released from soybean oil-containing preparations within the first five hours. We conclude from our results that an abraded surface of Dox-loaded drug delivery system increases their efficacy. The apparent match between surface geometry of drug delivery systems and target cells is suggested as a steppingstone for refined development of drug delivery systems. This is the first study to provide a systematic approach to developing next-generation drug carrier systems using structural insights to guide the development of next-generation drug delivery systems with increased efficacy and reduced side effects.
