EFFECT OF ALKOHOLIC EXTRACTS OF CHAMOMILE (Matricaria chamomilla L.) CALLUS IN INITIATION, GROWTH AND PROTEIN CONTENT OF BLACK BEAN (Nigella sativa L.)STEMS CALLUS

Abstract

The study was carried out to know the effect of alkoholic extracts of chamomile (Matricaria chamomilla L.) in callus initiation and growth of explants of black bean (Nigella sativa L.) ,apart from the protein content. Results cleared different response of explant to callus initiation and growth with different concentrations of alkoholic extracts of chamomile . Which had been added to the standard medium of black bean callus initiation and growth ] MS+0.221mg/L 2,4-D (2,4- Dichchlorophenoxy acetic acid) [ .The results refer when adding of 5 mg/ L of chamomile alkoholic extracts to standard MS medium was the best in initiation and growth the callus after 60 days with fresh weight average 12.11 g as comparing with 6.35 g of the standard medium with increasing in total content at 1.21 mg /gm as comparing with 0.61 mg /gm of standard medium ,and when adding chamomile alkoholic extracts alone to MS medium enhanced with degree initiation and growth callus..And also showed that adding of 5 mg /L alkoholic extracts to MS medium greatly enhanced callus initiation and growtg after 60 days with fresh weight average 8.54g with protein content 0.88 mg /gm as comparing with MS medium alone.