Evolution of gene knockout strains of E. coli reveal regulatory architectures governed by metabolism

Douglas McCloskey; Sibei Xu; Troy E. Sandberg; Elizabeth Brunk; Ying Hefner; Richard Szubin; Adam M. Feist; Bernhard O. Palsson

doi:10.1038/s41467-018-06219-9

Nature Communications (Sep 2018)

Evolution of gene knockout strains of E. coli reveal regulatory architectures governed by metabolism

Douglas McCloskey,
Sibei Xu,
Troy E. Sandberg,
Elizabeth Brunk,
Ying Hefner,
Richard Szubin,
Adam M. Feist,
Bernhard O. Palsson

Affiliations

Douglas McCloskey: Department of Bioengineering, University of California–San Diego
Sibei Xu: Department of Bioengineering, University of California–San Diego
Troy E. Sandberg: Department of Bioengineering, University of California–San Diego
Elizabeth Brunk: Department of Bioengineering, University of California–San Diego
Ying Hefner: Department of Bioengineering, University of California–San Diego
Richard Szubin: Department of Bioengineering, University of California–San Diego
Adam M. Feist: Department of Bioengineering, University of California–San Diego
Bernhard O. Palsson: Department of Bioengineering, University of California–San Diego

DOI: https://doi.org/10.1038/s41467-018-06219-9
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 15

Abstract

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The function of metabolic genes in the context of regulatory networks is not well understood. Here, the authors investigate the adaptive responses of E. coli after knockout of metabolic genes and highlight the influence of metabolite levels in the evolution of regulatory function.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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