Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola

Nicolas Kieffer; Nicolas Kieffer; Laurent Poirel; Laurent Poirel; Laurent Poirel; Claudine Fournier; Claudine Fournier; Brad Haltli; Russel Kerr; Patrice Nordmann; Patrice Nordmann; Patrice Nordmann; Patrice Nordmann

doi:10.3389/fmicb.2019.01673

Frontiers in Microbiology (Jul 2019)

Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola

Nicolas Kieffer,
Nicolas Kieffer,
Laurent Poirel,
Laurent Poirel,
Laurent Poirel,
Claudine Fournier,
Claudine Fournier,
Brad Haltli,
Russel Kerr,
Patrice Nordmann,
Patrice Nordmann,
Patrice Nordmann,
Patrice Nordmann

Affiliations

Nicolas Kieffer: Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland
Nicolas Kieffer: Department of Medicine, INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland
Laurent Poirel: Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland
Laurent Poirel: Department of Medicine, INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland
Laurent Poirel: Department of Medicine, Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland
Claudine Fournier: Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland
Claudine Fournier: Department of Medicine, INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland
Brad Haltli: Department of Biomedical Sciences, Atlantic Veterinary College, Charlottetown, PE, Canada
Russel Kerr: Department of Biomedical Sciences, Atlantic Veterinary College, Charlottetown, PE, Canada
Patrice Nordmann: Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland
Patrice Nordmann: Department of Medicine, INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland
Patrice Nordmann: Department of Medicine, Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland
Patrice Nordmann: Department of Medicine, Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland

DOI: https://doi.org/10.3389/fmicb.2019.01673
Journal volume & issue: Vol. 10

Abstract

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The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species Pseudobacteriovorax antillogorgiicola. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM-1, its closest relative. Kinetic parameters performed on purified PAN-1 showed it displayed a hydrolytic activity toward most β-lactams including carbapenems but spared cefepime and aztreonam. These results further highlight that environmental bacterial species may be reservoirs of MβL encoding genes.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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