Frontiers in Chemistry (Aug 2024)

Synthesis of BODIPY FL-tethered ridaifen-B, RID-B-BODIPY, and its localization in cancer cells

  • Takatsugu Murata,
  • Kyoka Komukai,
  • Yuta Semba,
  • Eri Murata,
  • Fumi Sato,
  • Tomohiro Takano,
  • Kaho Tsuchiya,
  • Chihiro Matsuda,
  • Anju Sakai,
  • Amane Yoneoka,
  • Shunsuke Takahashi,
  • Yukitoshi Nagahara,
  • Isamu Shiina

DOI
https://doi.org/10.3389/fchem.2024.1451468
Journal volume & issue
Vol. 12

Abstract

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We synthesized ridaifen-B boron dipyrromethene (RID-B-BODIPY) using 2-methyl-6-nitro benzoic anhydride (MNBA)-mediated dehydration condensation reaction between amino alkyl-tethered RID and BODIPY FL. Comparative experiments between dicyclohexylcarbodiimide (DCC) and MNBA for their coupling reactions demonstrated that MNBA is an effective condensation reagent for amines and BODIPY FL. A cell staining study with RID-B-BODIPY showed intracellular localization of BODIPY FL fluorescence, attributed to the RID-B structure, indicating the successful development of a tool for analyzing intracellular molecular behavior efficiently.

Keywords