Xin yixue (Jan 2022)

Effect of overexpression of forkhead box B2 on proliferation, invasion and migration of hepatocellular carcinoma cells

  • Liang Junhui, Chen Zerui, Peng Shuming, Chen Shanjia, Shu Bairong

DOI
https://doi.org/10.3969/j.issn.0253-9802.2022.01.010
Journal volume & issue
Vol. 53, no. 1
pp. 41 – 45

Abstract

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Objective To evaluate the effect of forkhead box protein B2 （FOXB2） on the proliferation, invasion and migration of hepatocellular carcinoma cells. Methods The expression levels of FOXB2 mRNA in normal liver cells LO2, hepatocellular carcinoma cells Hep3B and Huh7 were detected by quantitative PCR. The cell model overexpressing FOXB2 was constructed. The overexpression efficiency was assessed by real-time fluorescent quantitative PCR and Western blot. The effect of FOXB2 on Huh7 cell proliferation was evaluated by cell counting kit-8 （CCK-8）. The effect of FOXB2 on the clone formation of Huh7 cells was determined by plate cloning assay. The effect of FOXB2 on the invasion and migration of Huh7 cells was detected by Transwell chamber test. Results The expression levels of FOXB2 in hepatocellular carcinoma cells Huh7 and Hep3B were significantly lower than that in normal liver cells LO2 （both P<0.001）. Overexpression of FOXB2 significantly inhibited the proliferation （P=0.005） and clone formation （P<0.001） of Huh7 cells. In the Huh7-FOXB2 overexpression group, the invasion （P=0.002） and migration capabilities （P<0.001） of hepatocellular carcinoma cells were significantly lower than that in the Vector control cells. Conclusions FOXB2 is lowly expressed in hepatocellular carcinoma cells. Overexpression of FOXB2 suppresses the proliferation, invasion and migration of hepatocellular carcinoma cells.

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