Molecules (May 2019)

Simvastatin Attenuates H<sub>2</sub>O<sub>2</sub>-Induced Endothelial Cell Dysfunction by Reducing Endoplasmic Reticulum Stress

  • Zhiqiang He,
  • Xuanhong He,
  • Menghan Liu,
  • Lingyue Hua,
  • Tian Wang,
  • Qian Liu,
  • Lai Chen,
  • Nianlong Yan

DOI
https://doi.org/10.3390/molecules24091782
Journal volume & issue
Vol. 24, no. 9
p. 1782

Abstract

Read online

Atherosclerosis is the pathological basis of cardiovascular disease, whilst endothelial dysfunction (ED) plays a primary role in the occurrence and development of atherosclerosis. Simvastatin has been shown to possess significant anti-atherosclerosis activity. In this study, we evaluated the protective effect of simvastatin on endothelial cells under oxidative stress and elucidated its underlying mechanisms. Simvastatin was found to attenuate H2O2-induced human umbilical vein endothelial cells (HUVECs) dysfunction and inhibit the Wnt/β-catenin pathway; however, when this pathway was activated by lithium chloride, endothelial dysfunction was clearly enhanced. Further investigation revealed that simvastatin did not alter the expression or phosphorylation of LRP6, but reduced intracellular cholesterol deposition and inhibited endoplasmic reticulum (ER) stress. Inducing ER stress with tunicamycin activated the Wnt/β-catenin pathway, whereas reducing ER stress with 4-phenylbutyric acid inhibited it. We hypothesize that simvastatin does not affect transmembrane signal transduction in the Wnt/β-catenin pathway, but inhibits ER stress by reducing intracellular cholesterol accumulation, which blocks intracellular signal transduction in the Wnt/β-catenin pathway and ameliorates endothelial dysfunction.

Keywords