Metabolite Profiling, Antioxidant and Key Enzymes Linked to Hyperglycemia Inhibitory Activities of <i>Satureja hispidula</i>: An Underexplored Species from Algeria
Ammar Haouat,
Habiba Rechek,
Diana C. G. A. Pinto,
Susana M. Cardoso,
Mónica S. G. A. Válega,
Abdelhamid Boudjerda,
Artur M. S. Silva,
Ratiba Mekkiou
Affiliations
Ammar Haouat
Unité de Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL), Université des Frères Mentouri, Constantine 25000, Algeria
Habiba Rechek
LAQV-REQUIMTE & Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
Diana C. G. A. Pinto
LAQV-REQUIMTE & Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
Susana M. Cardoso
LAQV-REQUIMTE & Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
Mónica S. G. A. Válega
LAQV-REQUIMTE & Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
Abdelhamid Boudjerda
Laboratory of Cellular and Molecular Biology, University of Mohammed Seddik Benyahia, Jijel 18000, Algeria
Artur M. S. Silva
LAQV-REQUIMTE & Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
Ratiba Mekkiou
Unité de Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL), Université des Frères Mentouri, Constantine 25000, Algeria
In the present study, two extracts from the aerial parts of the endemic species Satureja hispidula were analyzed for the first time by ultra-high-performance liquid chromatography coupled with a diode array detector and an electrospray mass spectrometer (UHPLC-DAD-ESI/MS) method in order to identify and quantify their phenolic compounds. These extracts’ antioxidant, α-glucosidase and α-amylase inhibitory activities were also evaluated. UHPLC-DAD-ESI/MS allowed the identification of 28 and 20 compounds in the ethanolic and aqueous extracts, respectively; among them, 5-O-caffeoylquinic acid was the most abundant in both extracts. The biological assay results indicate that the species S. hispidula, besides its high antioxidant power, is also potentially useful for inhibiting the α-glucosidase enzyme. In both antioxidant and α-glucosidase inhibitory assays, the aqueous extract exhibited the most promising results, significantly better than the standards used as positive controls.
