Detection and Genotyping of Human Papillomavirus (HPV16/18), Epstein–Barr Virus (EBV), and Human Cytomegalovirus (HCMV) in Endometrial Endometroid and Ovarian Cancers
Beniamin Oskar Grabarek,
Piotr Ossowski,
Justyna Czarniecka,
Mateusz Ożóg,
Justyna Prucnal,
Ireneusz Dziuba,
Aleksander Ostenda,
Konrad Dziobek,
Dariusz Boroń,
Wojciech Peszek,
Piotr Kras,
Szymon Januszyk,
Maciej Dąbala,
Tomasz Kasela,
Marcin Opławski
Affiliations
Beniamin Oskar Grabarek
Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, Poland
Piotr Ossowski
Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, Poland
Justyna Czarniecka
Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, Poland
Mateusz Ożóg
Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, Poland
Justyna Prucnal
Department of Microbiology, SP ZOZ Szpital Wielospecjalistyczny w Jaworznie, 43-600 Jaworzno, Poland
Ireneusz Dziuba
Department of Pathology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, Poland
Aleksander Ostenda
Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, Poland
Konrad Dziobek
Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, Poland
Dariusz Boroń
Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, Poland
Wojciech Peszek
Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, Poland
Piotr Kras
Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, Poland
Szymon Januszyk
Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, Poland
Maciej Dąbala
Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, Poland
Tomasz Kasela
Department of Histology, Cytophysiology and Embryology, Faculty of Medicine, Academia of Silesia, 41-055 Zabrze, Poland
Marcin Opławski
Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, Poland
The purpose of this study was to evaluate the relationship between human papillomavirus (HPV16/18), Epstein–Barr virus (EBV), and human cytomegalovirus (HCMV) infections and the occurrence of ovarian cancer in 48 women, of whom 36 underwent surgery and chemotherapy (group A), 12 in whom surgery was sufficient (group B), and 60 with endometroid endometrial cancer stage G1-G3 (group C), compared to patients in whom the uterus and its appendages were removed for nononcological reasons (control group). The detection of HPV, EBV, and HCMV in tumor tissue and normal tissue was performed using the real-time polymerase chain reaction (RT-PCR) technique. A statistically significantly higher risk of endometrial cancer was noted in patients infected only with HCMV (OR > 1; p 1; p 1; p < 0.05). The obtained results suggest that HCMV infection is associated with the development of a stage of ovarian cancer when treatment can be completed with surgery alone. Meanwhile, EBV appears to be responsible for the development of ovarian cancer in more advanced stages.
