The Attraction of Apple Rhizosphere Microorganisms and Changing Leaf Characteristics by Strigolactone

Abstract

Apple leaf characteristics are the most important vegetative growth and development traits. The apple rhizosphere is also indirectly responsible for apple growth and development. It provides roots with elements and compounds that improve roots’ and shoots’ growth and development. The application of exogenous plant growth regulators such as strigolactones (SLs) has become one of the main trends for improving vegetative growth and enhancing the rhizosphere microbiome. This study aimed to investigate the effect of the exogenous SLs on some leaf characteristics, such as leaf area, angle, nitrogen content, chlorophyll content, and apical area diameter, in addition to the composition of the rhizosphere microbiome of apple M9 rootstock. The apple rootstocks were treated with various concentrations of GR24, an analog of SLs; the concentrations were 0, 1, 5, and 10 µM. The study found that the treatments of 5 µM increased the leaf–stem angles and leaf length while decreasing the apical diameter. The treatments of 1 and 5 µM increased leaf nitrogen content; however, this effect was not observed when using the higher concentration of 10 µM. The lower concentration (1 µM) led to a different abundance and diversity of microorganisms compared to the higher concentration (10 µm).

Keywords

• strigolactones (SLs)
• rhizosphere microbiome
• apple rootstock
• nitrogen content
• chlorophyll content
• apical area