Datasets depicting mobility retardation of NCS proteins observed upon incubation with calcium, but not with magnesium, barium or strontium
Jeffrey Viviano,
Anuradha Krishnan,
Jenna Scully,
Hao Wu,
Venkat Venkataraman
Affiliations
Jeffrey Viviano
Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ 08084, USA
Anuradha Krishnan
Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ 08084, USA
Jenna Scully
School of Osteopathic Medicine, Rowan University, Stratford, NJ 08084, USA
Hao Wu
Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ 08084, USA
Venkat Venkataraman
Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ 08084, USA; School of Osteopathic Medicine, Rowan University, Stratford, NJ 08084, USA; Corresponding author at: Graduate School of Biomedical Sciences, Rowan School of Osteopathic Medicine, Stratford, NJ 08084, USA.
In this data article we show the specificity of the Ca2+-induced mobility shift in three proteins that belong to the neuronal calcium sensor (NCS) protein family: Hippocalcin, GCAP1 and GCAP2. These proteins did not display a shift in mobility in native gels when incubated with divalent cations other than Ca2+ – such as Mg2+, Ba2+, and Sr2+, even at 10× concentrations. The data is similar to that obtained with another NCS protein, neurocalcin delta (Viviano et al., 2016, “Electrophoretic Mobility Shift in Native Gels Indicates Calcium-dependent Structural Changes of Neuronal Calcium Sensor Proteins”, [1]).
