Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.1290A > G (p.T351A) or c.2067A > G (p.T610A) in the RHOT1 gene encoding Miro1

Axel Chemla; Giuseppe Arena; Claudia Saraiva; Clara Berenguer-Escuder; Dajana Grossmann; Anne Grünewald; Christine Klein; Philip Seibler; Jens C. Schwamborn; Rejko Krüger

Stem Cell Research (Jun 2023)

Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.1290A > G (p.T351A) or c.2067A > G (p.T610A) in the RHOT1 gene encoding Miro1

Axel Chemla,
Giuseppe Arena,
Claudia Saraiva,
Clara Berenguer-Escuder,
Dajana Grossmann,
Anne Grünewald,
Christine Klein,
Philip Seibler,
Jens C. Schwamborn,
Rejko Krüger

Affiliations

Axel Chemla: Translational Neuroscience, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg
Giuseppe Arena: Translational Neuroscience, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg; Corresponding authors.
Claudia Saraiva: Developmental and Cellular Biology, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg
Clara Berenguer-Escuder: Translational Neuroscience, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg
Dajana Grossmann: Translational Neuroscience, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg; Translational Neurodegeneration Section “Albrecht-Kossel”, Department of Neurology, University Medical Center Rostock, University of Rostock, Rostock, Germany
Anne Grünewald: Institute of Neurogenetics, University of Lübeck, Lübeck, Germany; Molecular and Functional Neurobiology, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg
Christine Klein: Institute of Neurogenetics, University of Lübeck, Lübeck, Germany
Philip Seibler: Institute of Neurogenetics, University of Lübeck, Lübeck, Germany
Jens C. Schwamborn: Developmental and Cellular Biology, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg
Rejko Krüger: Translational Neuroscience, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg; Transversal Translational Medicine, Luxembourg Institute of Health (LIH), Luxembourg; Parkinson Research Clinic, Centre Hospitalier de Luxembourg (CHL), Luxembourg; Corresponding authors.

Journal volume & issue: Vol. 69
p. 103085

Abstract

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Primary skin fibroblasts from two Parkinson’s disease (PD) patients carrying distinct heterozygous mutations in the RHOT1 gene encoding Miro1, namely c.1290A > G (Miro1 p.T351A) and c.2067A > G (Miro1 p.T610A), were converted into induced pluripotent stem cells (iPSCs) by episomal reprogramming. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. Here, we provide a comprehensive characterization and quality assurance of both isogenic pairs, which will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes).

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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