Resonance energy transfer sensitises and monitors in situ switching of LOV2-based optogenetic actuators

Li-Li Li; Florence M. Klein; Lorenzo Li Greci; Arkadiusz Popinigis; Florian Freudenberg; Michael J. Courtney

doi:10.1038/s41467-020-18816-8

Nature Communications (Oct 2020)

Resonance energy transfer sensitises and monitors in situ switching of LOV2-based optogenetic actuators

Li-Li Li,
Florence M. Klein,
Lorenzo Li Greci,
Arkadiusz Popinigis,
Florian Freudenberg,
Michael J. Courtney

Affiliations

Li-Li Li: Neuronal Signalling Lab, Turku Bioscience Centre, University of Turku and Åbo Academy University
Florence M. Klein: Neuronal Signalling Lab, Turku Bioscience Centre, University of Turku and Åbo Academy University
Lorenzo Li Greci: Neuronal Signalling Lab, Turku Bioscience Centre, University of Turku and Åbo Academy University
Arkadiusz Popinigis: Neuronal Signalling Lab, Turku Bioscience Centre, University of Turku and Åbo Academy University
Florian Freudenberg: Department of Psychiatry, Psychosomatic Medicine and Psychotherapy, University Hospital, Goethe University
Michael J. Courtney: Neuronal Signalling Lab, Turku Bioscience Centre, University of Turku and Åbo Academy University

DOI: https://doi.org/10.1038/s41467-020-18816-8
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 18

Abstract

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Cellular optogenetics applications are limited by difficulties in quantification and blue light toxicity. Here the authors design LOV2-based switches that use resonance energy transfer to overcome these concerns.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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