Research of cGMP- and cAMP-dependent intracellular signaling systems in the insulin-mediated regulation of platelets aggregation activity in patients with heart failure and metabolic disturbances

Abstract

Data of the study of cGMP- and cAMP-dependent intracellular signaling systems in the insulin-mediated regulation of the platelet aggregation activity in 25 patients with DM2 and heart failure are presented. 15 healthy volunteers were recruited as a control group. To estimate cAMP- and cGMP-dependent intracellular signaling systems in the realization of insulin antiaggregational effect we used 3-isobutyl-1-methyl-xanthine — inhibitor of the phosphodiesterase V and IV, forskolin — stimulator of adenylate cyclase, zaprinast — specific inhibitor of the phosphodiesterase V. Modifiers of the adenylate cyclase/cAMP system — IBMX and forskolin — had the most prominent effect on the reduction of the aggregation of the isolated platelets.

Keywords

• сахарный диабет
• сердечная недостаточность
• тромбоциты
• агрегация
• вторичные мессенджеры