Natural Products Produced in Culture by Biosynthetically Talented <i>Salinispora arenicola</i> Strains Isolated from Northeastern and South Pacific Marine Sediments
David E. Williams,
Kalindi D. Morgan,
Doralyn S. Dalisay,
Teatulohi Matainaho,
Elodie Perrachon,
Noemie Viller,
Maïlys Delcroix,
Jeanne Gauchot,
Haruka Niikura,
Brian O. Patrick,
Katherine S. Ryan,
Raymond J. Andersen
Affiliations
David E. Williams
Departments of Chemistry and Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
Kalindi D. Morgan
Departments of Chemistry and Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
Doralyn S. Dalisay
Center for Chemical Biology and Biotechnology (C2B2), Department of Biology, College of Liberal Arts, Sciences and Education, University of San Agustin, Iloilo 5000, Philippines
Teatulohi Matainaho
School of Medicine and Health Sciences, University of Papua New Guinea, Boroko P.O. Box 5623, NCD, Papua New Guinea
Elodie Perrachon
Departments of Chemistry and Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
Noemie Viller
Departments of Chemistry and Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
Maïlys Delcroix
Departments of Chemistry and Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
Jeanne Gauchot
Departments of Chemistry and Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
Haruka Niikura
Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
Brian O. Patrick
Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
Katherine S. Ryan
Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
Raymond J. Andersen
Departments of Chemistry and Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC V6T 1Z1, Canada
Laboratory cultures of two ‘biosynthetically talented’ bacterial strains harvested from tropical and temperate Pacific Ocean sediment habitats were examined for the production of new natural products. Cultures of the tropical Salinispora arenicola strain RJA3005, harvested from a PNG marine sediment, produced salinorcinol (3) and salinacetamide (4), which had previously been reported as products of engineered and mutated strains of Amycolatopsis mediterranei, but had not been found before as natural products. An S. arenicola strain RJA4486, harvested from marine sediment collected in the temperate ocean waters off British Columbia, produced the new aminoquinone polyketide salinisporamine (5). Natural products 3, 4, and 5 are putative shunt products of the widely distributed rifamycin biosynthetic pathway.
