NITRATE REDUCTASE ENZYME ACTIVITY AND ITS CORRELATION WITH SOYBEANS YIELD IN SATURATED SOIL CULTURE AND WATERING CULTIVATION IN TIDAL LAND

Abstract

This study aimed to determine the Activity of Nitrate Reductase (ANR) of soybean in Saturated Soil Culture (SSC) and Watering Cultivation (WC) in tidal land and its correlation with aspects of soybean yield of the Anjasmoro cultivar. Treatment applications were arranged in a randomized block design (RBD) with three replications. The height of standing water in the trench in the Saturated Soil Culture system (SSC) as a treatment in this study was 15 cm, 20 cm, and 25 cm from the soil surface. WC actors were selected from 3 farmer groups. Soybean seeds were planted in experimental plots measuring 3 m x 2 m with a spacing of 30 cm x 20 cm, according to the spacing used by farmers. SP36, KCl, and Urea fertilizers were given as basic fertilizers at the recommended dosage. Observation variables included: ANR, number of filled pods/plant, dry seed weight/100 seeds, and dry seed weight/plant. The results showed that the height of standing water in the ditch in SSC affected the ANR and soybean yield. There was no difference in ANR for the height of the water in the ditch 15 cm and 20 cm from the soil surface, 12.76 mol NO2, respectively g-1hour-1 and 13.43 mol NO2. g-1. hour-1. The lowest ANR was obtained at the treatment level with the height of the puddle in the ditch 25 cm from the soil surface, namely 11.52 mol NO2. g-1.h-1, but still higher than the ANR in WC. There was no difference in the number of filled pods/plant, dry seed weight/100 seeds, and dry seed weight/plant between the height of standing water in the 15 cm trench and 20 cm from the soil surface.

Keywords

• nitrate reductase
• saturated soil culture
• soybean
• tidal lands