A distinct mammalian disome collision interface harbors K63-linked polyubiquitination of uS10 to trigger hRQT-mediated subunit dissociation

Momoko Narita; Timo Denk; Yoshitaka Matsuo; Takato Sugiyama; Chisato Kikuguchi; Sota Ito; Nichika Sato; Toru Suzuki; Satoshi Hashimoto; Iva Machová; Petr Tesina; Roland Beckmann; Toshifumi Inada

doi:10.1038/s41467-022-34097-9

Nature Communications (Oct 2022)

A distinct mammalian disome collision interface harbors K63-linked polyubiquitination of uS10 to trigger hRQT-mediated subunit dissociation

Momoko Narita,
Timo Denk,
Yoshitaka Matsuo,
Takato Sugiyama,
Chisato Kikuguchi,
Sota Ito,
Nichika Sato,
Toru Suzuki,
Satoshi Hashimoto,
Iva Machová,
Petr Tesina,
Roland Beckmann,
Toshifumi Inada

Affiliations

Momoko Narita: Division of RNA and gene regulation, Institute of Medical Science, The University of Tokyo
Timo Denk: Gene Center and Department of Biochemistry, University of Munich
Yoshitaka Matsuo: Division of RNA and gene regulation, Institute of Medical Science, The University of Tokyo
Takato Sugiyama: Graduate School of Pharmaceutical Sciences, Tohoku University
Chisato Kikuguchi: Division of RNA and gene regulation, Institute of Medical Science, The University of Tokyo
Sota Ito: Division of RNA and gene regulation, Institute of Medical Science, The University of Tokyo
Nichika Sato: Division of RNA and gene regulation, Institute of Medical Science, The University of Tokyo
Toru Suzuki: Division of RNA and gene regulation, Institute of Medical Science, The University of Tokyo
Satoshi Hashimoto: Graduate School of Pharmaceutical Sciences, Tohoku University
Iva Machová: Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague
Petr Tesina: Gene Center and Department of Biochemistry, University of Munich
Roland Beckmann: Gene Center and Department of Biochemistry, University of Munich
Toshifumi Inada: Division of RNA and gene regulation, Institute of Medical Science, The University of Tokyo

DOI: https://doi.org/10.1038/s41467-022-34097-9
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

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Collided ribosomes are marked by ubiquitination to induce quality control mechanisms. Here, authors show that mammalian disomes form a distinct structural interface, in which uS10 K63-linked polyubiquitination is critical for ribosome dissociation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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