Following replicative DNA synthesis by time-resolved X-ray crystallography

Nicholas Chim; Roman A. Meza; Anh M. Trinh; Kefan Yang; John C. Chaput

doi:10.1038/s41467-021-22937-z

Nature Communications (May 2021)

Following replicative DNA synthesis by time-resolved X-ray crystallography

Nicholas Chim,
Roman A. Meza,
Anh M. Trinh,
Kefan Yang,
John C. Chaput

Affiliations

Nicholas Chim: Department of Pharmaceutical Sciences, University of California
Roman A. Meza: Department of Pharmaceutical Sciences, University of California
Anh M. Trinh: Department of Pharmaceutical Sciences, University of California
Kefan Yang: Department of Chemical and Biomolecular Engineering, University of California
John C. Chaput: Department of Pharmaceutical Sciences, University of California

DOI: https://doi.org/10.1038/s41467-021-22937-z
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 8

Abstract

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DNA polymerases are the key enzymes responsible for DNA replication and repair. Here the authors reveal through time-lapsed images of X-ray crystal structures that translocation precedes phosphodiester bond formation in the mechanism of DNA synthesis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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