Food and Agricultural Immunology (Jan 2018)

Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody

  • Jiajia Sun,
  • Liqiang Liu,
  • Shanshan Song,
  • Gang Cui,
  • Hua Kuang

DOI
https://doi.org/10.1080/09540105.2018.1490394
Journal volume & issue
Vol. 29, no. 1
pp. 930 – 940

Abstract

Read online

The problem of waste oil is a food safety issue that requires urgent resolution. In this study, a novel hapten was designed for the rapid detection of capsaicinoids, a marker of waste oil, and a monoclonal antibody (MAb) 5D7 was generated that is specific for three major capsaicinoids: capsaicin (CPC), dihydrocapsaicin (DCPC), and N-vanillylnonanamide (N-V). The half maximal inhibitory concentrations of MAb 5D7 for CPC, DCPC, and N-V were 1.12, 0.8, and 0.87 ng/mL, respectively. An immunochromatographic strip test based on MAb 5D7 was established for these three capsaicinoids, with visual inspection limits of <1.0 ng/mL, which satisfies the requirement for the rapid on-site inspection of waste oil.

Keywords