A step‐by‐step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited

Abstract

Premise Counting chromosomes is a fundamental botanical technique, yet it is often intimidating and increasingly sidestepped. Once mastered, the basic protocol can be applied to a broad range of taxa and research questions. It also reveals an aspect of the plant genome that is accessible with only the most basic of resources—access to a microscope with 1000× magnification is the most limiting factor. Methods and Results Here we provide a detailed protocol for choosing, staining, and squashing angiosperm pollen mother cells. The protocol is supplemented by figures and two demonstration videos. Conclusions The protocol we provide will hopefully demystify and reinvigorate a powerful and once commonplace botanical technique that is available to researchers regardless of their location and resources.

Keywords

• chromosome number
• chromosome squash
• cytology
• meiosis
• polyploidy
• species complex