Effect of Calcium Silicate and β-Tricalcium Phosphate Reinforcement on the Mechanical–Biological Properties of Freeze-Dried Collagen Composite Scaffolds for Bone Tissue Engineering Applications
Temesgen Yiber Animut,
Henni Setia Ningsih,
Hsueh-Huan Shih,
Meng-Huang Wu,
Shao-Ju Shih
Affiliations
Temesgen Yiber Animut
Department of Materials Science and Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Road, Taipei 10607, Taiwan
Henni Setia Ningsih
Department of Fragrance and Cosmetic Science, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan
Hsueh-Huan Shih
Department of Materials Science and Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Road, Taipei 10607, Taiwan
Meng-Huang Wu
Department of Orthopedics, Taipei Medical University Hospital, Taipei 11031, Taiwan
Shao-Ju Shih
Department of Materials Science and Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Road, Taipei 10607, Taiwan
The development of a collagen-based composite scaffold to repair damaged bone is one of many important issues in tissue engineering. In this study, pure collagen, collagen/β-tricalcium phosphate (β-TCP), collagen/calcium silicate (CS), and collagen/β-TCP/CS scaffolds were fabricated using the freeze-drying method. The phase compositions, microstructures, and mechanical properties were characterized using X-ray diffraction, scanning electron microscopy, and a universal testing machine, respectively. In addition, cell viability was evaluated using an MTT assay. Finally, the correlations between the density, mechanical properties, and biodegradation behaviors of pore size distributions were discussed.
