Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals

Xiaogang Xu; Manisha Pradhan; Miao Xu; Yu-Shan Cheng; Jeanette Beers; Kaari L. Linask; Yongshun Lin; Wei Zheng; Jizhong Zou

Stem Cell Research (Dec 2020)

Four induced pluripotent stem cell lines (TRNDi021-C, TRNDi023-D, TRNDi024-D and TRNDi025-A) generated from fibroblasts of four healthy individuals

Xiaogang Xu,
Manisha Pradhan,
Miao Xu,
Yu-Shan Cheng,
Jeanette Beers,
Kaari L. Linask,
Yongshun Lin,
Wei Zheng,
Jizhong Zou

Affiliations

Xiaogang Xu: iPSC Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA; Zhejiang Provincial Key Lab of Geriatrics & Geriatrics Institute of Zhejiang Province, Department of Geriatrics, Zhejiang Hospital, Hangzhou 310013, China
Manisha Pradhan: National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA
Miao Xu: National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA
Yu-Shan Cheng: National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA
Jeanette Beers: iPSC Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Kaari L. Linask: iPSC Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Yongshun Lin: iPSC Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Wei Zheng: National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA
Jizhong Zou: iPSC Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA; Corresponding author.

Journal volume & issue: Vol. 49
p. 102011

Abstract

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Expanded human skin fibroblast cells from four different aged healthy individuals, 11-year-old female, 1-year-old male, 2-month-old male, and 8-year-old male, were used to generate integration-free induced pluripotent stem cell (iPSC) lines TRNDi021-C, TRNDi023-D, TRNDi024-D, and TRNDi025-A, respectively, by exogenous expression of four reprogramming factors, human SXO2, OCT3/4, C-MYC, KLF4. The authenticity of established iPSC lines was confirmed by the expressions of stem cell markers, karyotype analysis, and teratoma formation. These iPSC lines could serve as young healthy controls for the studies involving patient-specific iPSCs.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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