Detection of Rice stripe virus in Laodelphax striatellus by direct dot immunobinding assay

Abstract

Three horseradish peroxidase-linked monoclonal antibodies of Rice stripe virus (RSV), HRP-3B9, HRP-2H2 and HRP-2E5, were produced by NaIO4 method. The diluted times of the three enzyme-linked antibodies were 1∶25600, 1∶1600 and 1∶3200, respectively. In direct ELISA, the suitable diluted times of HRP-3B9 was 1∶5000. Enzyme-linked antibody HRP-3B9 was selected to detect RSV in Laodelphax striatellus Fallen with direct dot immunobinding assay (DIBA) and indirect DIBA, and the detection results showed that the direct DIBA was more sensitive than indirect DIBA. The direct DIBA was then used to detect the virus in Laodelphax striatellus Fallen collected from 10 counties in Jiangsu province, China.

Keywords

• <italic>Rice stripe virus</italic>
• DIBA
• Enzyme-linked antibodies
• <italic>Laodelphax striatellus</italic>