Phenotypic, molecular, and virulence characterization of entomopathogenic fungi, Beauveria bassiana (Balsam) Vuillemin, and Metarhizium anisopliae (Metschn.) Sorokin from soil samples of Ethiopia for the development of mycoinsecticide

Abstract

Fungal entomopathogens are the most effective biocontrol agents against insect pests in the natural ecosystem. This study was conducted for phenotypic, molecular, and virulence characterization of locally isolated entomopathogenic fungi from soil samples of six localities in Ethiopia. Entomopathogenic fungi were isolated from 120 soil samples with the galleria baiting method. A total of 65 (54.2%) entomopathogenic fungal isolates belongs to Beauveria spp and Metarhizium spp were identified based on cultural and morphological features. All isolates were pre-screened based on germination, vegetative growth rate, and spore production as fungal in-vitro virulence determinates. Isolate AAUKB-11 displayed the peak germination of 99.67% and isolate AAUMFB-77 achieved the highest radial growth rate of 3.43 mm day−1 with the highest sporulation 4.60 × 108spores/ml. The phylogenetic analysis of ITS-rDNA confirmed that 7 isolates were identified as B. bassiana and 5 isolates were categorized into M. anisopliae. Selected B. bassiana and M. anisopliae strains were evaluated for their pathogenicity efficiency against G. mellonella larvae and caused 86.67%–100% mortality. The mortality rates of G. mellonella larvae peaked at 100% with 4(33.33%) isolates from B. bassiana and 2(16.67%) isolates from M. anisopliae after 10 days of treatments. The high virulent isolate, B. bassiana AAUMB-29 displayed the least LT50 value of 2.36 days followed by isolate B. bassiana AAUMFB-77 with LT50 of 2.53 days. Future studies should be needed to focus on the evaluation of high virulent isolates against other potential insect pests to assess their vigorous role as favorable biological control agents.

Keywords

• Entomopathogenic fungi
• Pathogenicity
• G. mellonella
• B. bassiana
• M. anisopliae