Plants (Apr 2023)

In Depth Topological Analysis of Arabidopsis Mid-SUN Proteins and Their Interaction with the Membrane-Bound Transcription Factor MaMYB

  • Bisa Andov,
  • Aurelia Boulaflous-Stevens,
  • Charlotte Pain,
  • Sarah Mermet,
  • Maxime Voisin,
  • Camille Charrondiere,
  • Emmanuel Vanrobays,
  • Sylvie Tutois,
  • David E. Evans,
  • Verena Kriechbaumer,
  • Christophe Tatout,
  • Katja Graumann

DOI
https://doi.org/10.3390/plants12091787
Journal volume & issue
Vol. 12, no. 9
p. 1787

Abstract

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Mid-SUN proteins are a neglected family of conserved type III membrane proteins of ancient origin with representatives in plants, animals, and fungi. Previous higher plant studies have associated them with functions at the nuclear envelope and the endoplasmic reticulum (ER). In this study, high-resolution confocal light microscopy is used to explore the localisation of SUN3 and SUN4 in the perinuclear region, to explore topology, and to study the role of mid-SUNs on endoplasmic reticulum morphology. The role of SUN3 in the ER is reinforced by the identification of a protein interaction between SUN3 and the ER membrane-bound transcription factor maMYB. The results highlight the importance of mid-SUNs as functional components of the ER and outer nuclear membrane.

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