Cancer Management and Research (Dec 2020)

MicroRNA-361-3p Inhibit the Progression of Lymphoma by the Wnt/β-Catenin Signaling Pathway

  • Zhou H,
  • Tang H,
  • Li N,
  • Chen H,
  • Chen X,
  • Gu L,
  • Zhang L,
  • Tian G,
  • Tao D

Journal volume & issue
Vol. Volume 12
pp. 12375 – 12384

Abstract

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Hui Zhou,1 Huifeng Tang,1 Ning Li,1 Hang Chen,1 Xiaohui Chen,1 Lei Gu,1 Liang Zhang,1 Guoyan Tian,1 Diehong Tao2 1Department of Hematology and Oncology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, People’s Republic of China; 2Hematology Department, Tongde Hospital, Zhejiang Province, Hangzhou 310003, People’s Republic of ChinaCorrespondence: Hui ZhouDepartment of Hematology and Oncology, The Affiliated Hospital of Hangzhou Normal University, 126# Wenzhou Road, Hangzhou 310015, People’s Republic of ChinaTel +86-571-88358150Email [email protected] TaoHematology Department, Tongde Hospital of Zhejiang Province, #345 Gucui Road, Hangzhou 310003, People’s Republic of ChinaTel +86-571-89972417Email [email protected]: MicroRNA is involved in the development of lymphoma. It is reported that miR-361-3p has a tumor inhibitory effect, but its role in lymphoma is still unclear. The purpose of this study is to examine whether miR-361-3p can inhibit the development of lymphoma and further explore the related potential mechanism.Methods: In this study, we first analyzed the biological function of miR-361-3p in transfected Raji that mimicked miRNA. We also analyzed the biological function of the whole population in stably expressed miR-361-3p transgenic cells. Next, we conducted a complete micro-gene network to test the genetic profile of differential expression of stable gene-modified cells.Results: We found that miR-361-3p expression was often reduced in lymphoma cell lines. Cellular assays have shown a significant role in inhibiting the growth of miR-361-3p by inhibiting lymphoma proliferation and migration, and severely inhibiting the Wnt/β-catenin series protein signal. Bioinformatics analysis shows that Wnt10A is a new target of miR-361-3p, which is confirmed by our mechanism research. It is confirmed that restoring Wnt10A can reduce the tumor inhibition of Wnt/β-catenin during lymphoma progression and restore the normal signal of Wnt/β-catenin series proteins.Discussion: Our data indicate that miR-361-3p inhibits the Wnt/β-catenin protein signal by locking Wnt10A, which is an important factor in inhibiting the tumor in the pathogenesis of lymphoma. The miR-361-3p/Wnt10A axis may be a promising target for the treatment of lymphoma.Keywords: miRNA, lymphoma, Wnt, pathway, tumor suppressor

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