Generation of SHMT2 knockout human embryonic stem cell line (WAe009-A-67) using CRISPR/Cas9 technique

Jisheng Li; Bowen Zhang; Min Gan; Yunxing Li; Lijuan He; Wen Yue; Haixuan Qiao; Xuetao Pei; Yanhua Li

Stem Cell Research (Dec 2021)

Generation of SHMT2 knockout human embryonic stem cell line (WAe009-A-67) using CRISPR/Cas9 technique

Jisheng Li,
Bowen Zhang,
Min Gan,
Yunxing Li,
Lijuan He,
Wen Yue,
Haixuan Qiao,
Xuetao Pei,
Yanhua Li

Affiliations

Jisheng Li: Stem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion Medicine, Beijing 100850, China
Bowen Zhang: Beijing Institute of Radiation Medicine, Beijing 100850, China; South China Research Center for Stem Cell & Regenerative Medicine, SCIB, Guangzhou 510005, China
Min Gan: Stem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion Medicine, Beijing 100850, China
Yunxing Li: Beijing Institute of Radiation Medicine, Beijing 100850, China
Lijuan He: Stem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion Medicine, Beijing 100850, China; South China Research Center for Stem Cell & Regenerative Medicine, SCIB, Guangzhou 510005, China
Wen Yue: Stem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion Medicine, Beijing 100850, China; South China Research Center for Stem Cell & Regenerative Medicine, SCIB, Guangzhou 510005, China
Haixuan Qiao: School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, China; Corresponding authors at: School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, China (H. Qiao); Stem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion Medicine, Beijing 100850, China (X. Pei); Beijing Institute of Radiation Medicine, Beijing 100850, China (Y. Li).
Xuetao Pei: Stem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion Medicine, Beijing 100850, China; South China Research Center for Stem Cell & Regenerative Medicine, SCIB, Guangzhou 510005, China; Corresponding authors at: School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, China (H. Qiao); Stem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion Medicine, Beijing 100850, China (X. Pei); Beijing Institute of Radiation Medicine, Beijing 100850, China (Y. Li).
Yanhua Li: Beijing Institute of Radiation Medicine, Beijing 100850, China; South China Research Center for Stem Cell & Regenerative Medicine, SCIB, Guangzhou 510005, China; Corresponding authors at: School of Biomedical Engineering and Technology, Tianjin Medical University, Tianjin 300070, China (H. Qiao); Stem Cell and Regenerative Medicine Lab, Institute of Health Service and Transfusion Medicine, Beijing 100850, China (X. Pei); Beijing Institute of Radiation Medicine, Beijing 100850, China (Y. Li).

Journal volume & issue: Vol. 57
p. 102581

Abstract

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Serine hydroxymethyltransferase 2 (SHMT2), a catalytic enzyme playing an important role in aerobic cellular respiration and mitochondrial metabolism, might be pivotal in self-renewal and differentiation of human pluripotent stem cells. Herein, we used the CRISPR/Cas9 editing system to construct a homozygous SHMT2 knockout (SHMT2-KO) human embryonic stem cell (hESC) line, exhibiting a normal karyotype, colony morphology, and high expression levels of pluripotent proteins. Furthermore, SHMT2 knockout did not impact the self-renewal ability or differentiation potential into three germ layers of hESCs. Accordingly, this cell line provides a valuable model for further assessing SHMT2 functions in human embryonic development.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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